Publish in this journal
Journal Information
Vol. 221. Issue 3.
Pages 139-144 (March 2021)
Share
Share
Download PDF
More article options
ePub
Visits
...
Vol. 221. Issue 3.
Pages 139-144 (March 2021)
Original article
DOI: 10.1016/j.rceng.2020.04.010
FluoroType® MTB in pleural fluid for diagnosing tuberculosis
FluoroType® MTB en líquido pleural para el diagnóstico de tuberculosis
Visits
...
S. Bielsaa, A. Bernetb, C. Civita, C. Acostaa, A. Manonellesb, J.M. Porcela,
Corresponding author
jporcelp@yahoo.es

Corresponding author.
a Unidad de Medicina Pleural, Servicio de Medicina Interna, Hospital Universitario Arnau de Vilanova, Instituto de Investigación Biomédica de Lleida, IRBLleida, Lérida, Spain
b Servicio de Microbiología, Hospital Universitario Arnau de Vilanova, Instituto de Investigación Biomédica de Lleida, IRBLleida, Lérida, Spain
Article information
Abstract
Full Text
Bibliography
Download PDF
Statistics
Figures (1)
Tables (4)
Table 1. Characteristics of the population.
Table 2. Biochemical characteristics of the pleural fluid in the study population.
Table 3. Efficacy measures of microbiological techniques in pleural fluid and sputum for diagnosing pleural tuberculosis.
Table 4. Complementarity of the FluoroType MTB® test with other microbiological data in patients with pleural tuberculosis.a
Show moreShow less
Abstract
Objectives

This work aims to investigate the diagnostic accuracy of a nucleic acid amplification test (FluoroType MTB®) in pleural fluid (PF) and sputum to diagnose tuberculous pleural effusion (TPE). We also analyzed the increase in diagnostic accuracy of a second FluoroType MTB® test on a second thoracentesis sample when the first was negative.

Methods

We conducted a prospective single-center study that included 207 patients with pleural effusion (31 tuberculous and 176 due to other causes). Of the 31 cases of TPE, 21 (68%) were confirmed histologically or microbiologically; the other cases were considered probable.

Results

The operational characteristics of FluoroType MTB® in PF for identifying tuberculosis were a sensitivity of 13%, a specificity of 99%, a positive likelihood ratio of 11, and a negative likelihood ratio of 0.9. The diagnostic efficacy data for sputum samples were 21%, 91%, 2.4, and 0.9, respectively. PF and sputum cultures in solid and liquid media had greater sensitivity (36% and 31%, respectively). A second FluoroType MTB® test in PF was negative for 24 patients who had TPE and for whom the first FluoroType MTB® test was also negative. Only two (6.5%) patients with TPE had a confirmed diagnosis based exclusively on the positive results of the FluoroType MTB® in PF.

Conclusion

Due to its low sensitivity, the FluoroType MTB® test in PF has a limited role in diagnosing tuberculous pleurisy.

Keywords:
FluoroType MTB
Pleural tuberculosis
Pleural effusion
Resumen
Objetivos

Evaluar la rentabilidad de una prueba de amplificación de ácidos nucleicos (FluoroType MTB®) en líquido pleural (LP) y esputo para diagnosticar derrame pleural tuberculoso (DPT). Adicionalmente, se analizó el aumento de la rentabilidad diagnóstica de una segunda prueba FluoroType MTB®, obtenida mediante nueva toracocentesis, cuando la primera había sido negativa.

Métodos

Estudio prospectivo de un único centro que incluyó 207 pacientes con derrame pleural (31 tuberculosos y 176 de otras causas). De los 31 DPT, 21 (68%) se confirmaron histológica o microbiológicamente, considerándose el resto de casos como probables.

Resultados

Las características operativas de FluoroType MTB® en LP para identificar tuberculosis fueron: sensibilidad 13%, especificidad 99%, likelihood ratio (LR) positiva 11 y LR negativa 0,9. Los datos de eficacia diagnóstica en muestras de esputo fueron 21%, 91%, 2,4 y 0,9, respectivamente. Los cultivos de LP y esputo en medios sólidos y líquidos tuvieron una sensibilidad superior (36% y 31%, respectivamente). Una segunda prueba de FluoroType MTB® en LP fue negativa en los 24 pacientes con DPT y una primera prueba de FluoroType MTB® también negativa. Sólo en 2 (6,5%) pacientes con DPT la confirmación diagnóstica se basó exclusivamente en los resultados positivos del FluoroType MTB® en LP.

Conclusión

Debido a su baja sensibilidad, la prueba FluoroType MTB® en LP tiene un papel limitado en el diagnóstico de pleuritis tuberculosa.

Palabras clave:
FluoroType MTB
Tuberculosis pleural
Derrame pleural

Article

These are the options to access the full texts of the publication Revista Clínica Española (English Edition)
Member
Si es usted socio de FESEMI siga los siguientes pasos:

Diríjase desde aquí a la web de la >>>FESEMI<<< e inicie sesión mediante el formulario que se encuentra en la barra superior, pulsando sobre el candado.

Una vez autentificado, en la misma web de FESEMI, en el menú superior, elija la opción deseada.

>>>FESEMI<<<

Subscriber
Subscriber

If you already have your login data, please click here .

If you have forgotten your password you can you can recover it by clicking here and selecting the option “I have forgotten my password”
Subscribe
Subscribe to

Revista Clínica Española (English Edition)

Purchase
Purchase article

Purchasing article the PDF version will be downloaded

Price 19.34 €

Purchase now
Contact
Phone for subscriptions and reporting of errors
From Monday to Friday from 9 a.m. to 6 p.m. (GMT + 1) except for the months of July and August which will be from 9 a.m. to 3 p.m.
Calls from Spain
932 415 960
Calls from outside Spain
+34 932 415 960
Email
Idiomas
Revista Clínica Española (English Edition)

Subscribe to our newsletter

Article options
Tools
es en

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?